Unbiased, Efficient Characterization of Metagenome Functions with PacBio HiFi Sequencing

October 29, 2019

Organized in collaboration with





  • Kellye Eversole, Executive Director, International Alliance for Phytobiomes Research
  • Meredith Ashby, Ph.D. Director, Market Strategy, Microbial Genomics Pacific Biosciences


Understanding interactions among plants and the complex communities of organisms living on, in and around them requires more than one experimental approach. A new method for de novo metagenome assembly, PacBio HiFi sequencing, has unique strengths for determining the functional capacity of metagenomes. With HiFi sequencing, the accuracy and median read length of unassembled data outperforms the quality metrics for many existing assemblies generated with other technologies, enabling cost-competitive recovery of full-length genes and operons even from rare species. When paired with the ability to close the genomes of even challenging isolates like Xanthomonas, the PacBio Sequel II System is a powerful tool for phytobiome research.

Attendees learned:
  1. The unique strengths of HiFi sequencing for metagenome assembly and gene discovery.
  2. The expected performance and estimated cost of metagenome sequencing on the PacBio Sequel II System.
  3. The details of the microbial whole genome sequencing pipeline on the Sequel II System, from experiment planning to assembly.